Molecular Biology

The Molecular Biology team is looking for a Research Associate with experience in recombinant DNA technology and microbial strain engineering. We are specifically looking for an energetic, creative, and highly self-motivated person that enjoys challenges in microbial strain development with the purpose of heterologous protein expression.

**Job description**:

- In silico design of DNA/plasmid constructs and execution of the lab-based recombinant DNA work (standard restriction enzyme cloning, Gibson cloning, Golden Gate cloning,)
- Strain engineering/transformation of yeast, filamentous fungi, bacterial systems.
- Evaluate generated strains via standard techniques such as PCR and (RT)-qPCR analysis, Southern blot,
- Evaluate protein expression potential of engineered strains via small-scale cultivation, followed by protein detection methods such as gel-based separation (e.g. SDS-PAGE), Western blot, ELISA,
- Execute the designed experiments in the lab, followed by data collection, data analysis and data interpretation.
- Execute all experiments according to the in-house established standard operating procedures.
- Participate in revision of existing and writing of new standard operating procedures.
- Collaborate with and help colleagues within the team for execution of experiments.
- Responsible for individual safety and that of your colleagues by following the correct procedures and policies.

**Your profile**:

- Bachelor/Master degree in Life Sciences, preferably with relevant work experience.
- Good insights in recombinant DNA technology, microbiology, microbial strain engineering, strain cultivation and analysis, protein analysis.
- Knowledge of vector cloning software (e.g. VectorNTI, Geneious) is a plus.
- Experience with heterologous protein expression in microbial systems is a plus.
- Experience with (or interest in) bioreactor cultivation of micro-organisms (yeast, fungi, bacteria) is an added value.
- Demonstrate ability to work independently as well as in team environment.
- Demonstrate problem-solving abilities and analytical skills.
- Flexible within a dynamic environment, able to multi-task.
- Communication and organizational skills (interpretation and documentation of data, report writing, planning).